The bioinformatics analysis of gene methylation for children steroid resistance nephrotic syndrome
10.3760/cma.j.issn.1001-7097.2016.10.006
- VernacularTitle:儿童激素抵抗型肾病综合征基因甲基化研究及生物信息学分析
- Author:
Shumin RUI
;
Chunlin GAO
;
Zhengkun XIA
;
Jiayi ZHANG
- Publication Type:Journal Article
- Keywords:
Nephritic syndrome;
Methylation;
Steroid resistance
- From:
Chinese Journal of Nephrology
2016;32(10):753-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective Through selecting abnormal DNA methylation of children steroid resistance nephrotic syndrome and bioinformatics analysis to find the pathogenesis of steroid resistance nephrotic syndrome and provide new targets for therapy. Methods We use illumine 450K methylation chip to detected blood gene DNA methylation of 9 cases of children primary nephrotic syndrome. 9 cases were divided into 2 groups: G1 is the group of steroid sensitive nephritic syndrome, a total of 4 cases; G2 is the group of steroid resistance nephrotic syndrome, a total of 5 cases. Selected the abnormal DNA methylation in children steroid resistant nephritic syndrome, clarified the function of those genes through using functional annotation of gene GO, enrichment analysis and KEGG pathway analysis, conducted the preliminary analysis on children with steroid resistant nephrotic syndrome of gene methylation. Results Compared with the control group, G2 has a number of genes that were extensively methylated. According to the results of bioinformatics analysis, the abnormal DNA methylation in G2 is the components of the various kinds of organelles and cell membrane. They also regulated the polymerization and composition of cytoskeleton and actin, as well as involved in the process of metabolism of many amino acids and drug. Conclusions The abnormal DNA methylation in the group 2 have extensive role, offering possibility of clinical prediction and provided potential therapeutic targets.
