Progress in laboratory diagnosis of heparin induced thrombocytopenia
10.3760/cma.j.issn.1009-9158.2016.10.017
- VernacularTitle:肝素诱导的血小板减少症的实验室诊断进展
- Author:
Jianlong MEN
;
Jing REN
- Publication Type:Journal Article
- Keywords:
Thrombocytopenia;
Heparin;
Autoantibodies;
Serotonin;
Platelet activation;
Enzyme-linked immunosorbent assay;
Nephelometry and turbidimetry;
Immunoassay
- From:
Chinese Journal of Laboratory Medicine
2016;39(10):795-800
- CountryChina
- Language:Chinese
-
Abstract:
Heparin induced thrombocytopenia ( HIT ) is a severe side effect of heparin with antibody-mediation.Laboratory assays can be divided into two major categories, about functional assays and HIT antibodiesdetection.Thefunctional assays, such as the serotonin release assay ( SRA ) and heparin-induced platelet activationassay( HIPA) , are sensitive and specific for HIT.They arethe reference standard assays generally, but have thedeficiencies of complicated operation and time-consuming, and cannot be used as a routine examination. TheHIT antibodiesdetections, such as ELISA, immune turbidimetry assay, chemiluminescent assay and lateral flow immunoassay, have high diagnostic sensitivityandareavailable at routine laboratories.They can exclude the diagnosis of HIT or beused to diagnose HIT effectively combined with the pre-test probability score(4Ts score) of HIT.
