Effect of gypenoside on lipopolysaccharide-mediated microglial inflammatory response
10.3760/cma.j.issn.1673-4165.2016.08.008
- VernacularTitle:绞股蓝皂苷对脂多糖诱导的小胶质细胞炎性反应的影响
- Author:
Xiaorong XUE
;
Bin HU
;
Zhaoju LI
;
Huichuan WANG
;
Hui MIN
;
Bei LI
;
Qi GUO
- Publication Type:Journal Article
- Keywords:
Microglia;
Gypenoside;
Lipopolysaccharides;
Inflammation;
Suppressor of Cytokine Signaling Proteins;
NF-κB;
Tumor Necrosis Factor-α;
Interleukins
- From:
International Journal of Cerebrovascular Diseases
2016;24(8):730-733
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of gypenoside on lipopolysaccharide (LPS)-mediated inflammatory response. Methods The BV2 microglia cell line was cultured in vitro. The BV2 microglia cells were divided into four groups: normal control, LPS (10 ng/ml), GP + LPS (GP 20 μg/ml, LPS 10 ng/ml), and GP (20 μg/ml). After 24 h cultivation, ELISA was used to detect the levels of tumor necrosis factor α(TNF-α), interleukin (IL)-1β, and IL-6. Immunocytochemistry staining and Western blot were used to detect the expression levels of nuclear factor (NF-κB) and suppressor of cytokine signaling 1 (SOCS-1). Results Compared with the normal control group, the release of TNF-α, IL-1β and IL-6, as well as the expression level of NF-κB in the LPS group were increased significantly (all P < 0. 001). Compared with the LPS group, the release of TNF-α, IL-1β and IL-6, as well as the expression level of NFκB were decreased significantly, while the expression level of SOCS-1 was increased significantly (P < 0. 001). There were no significant differences in the release of TNF-α, IL-1β and IL-6, as well as the expression levels of NF-κB and SOCS-1 between the GP group and normal control group (all P > 0. 05 ). Conclusions GP can significantly inhibit the LPS-mediated microglial inflammatory response. SOCS-1 protein may be involved in GP inhibiting LPS-mediated microglial inflammatory response.
