Application of Multiple Displacement Amplification in Samples with Inhibitors
10.3969/j.issn.1004-5619.2016.05.006
- VernacularTitle:多重置换扩增在含抑制物检材中的应用
- Author:
Dongxue DING
;
Mei DING
- Publication Type:Journal Article
- Keywords:
forensic genetics;
nucleic acid amplification techniques;
multiple displacement amplification;
inhibitors;
hemoglobin;
humic acid
- From:
Journal of Forensic Medicine
2016;32(5):342-345
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the ability of inhibition resistibility of multiple displacement amplification (MDA)in samples with inhibitors. To explain the application and value of MDA in forensic medicine by comparing with using magnetic beads methods(MBM)to purify sample.MethodsDifferent concentra-tions of hemoglobin and humid acid(HA)mixed with DNA samples and then divided the samples into MDA group, MBM group and control group.D3S1358locus was amplified and detected by polyacry-lamide gel electrophoresis detection system and AmpF?STR? IdentifilerTM Plus Kit-capillary electrophore-sis detection system.ResultsWhen hemoglobin concentrations exceed 1 ng/μL or HA concentrations ex-ceed 0.1 ng/μL, amplification products could not be obtained by single-locus system in control group. When hemoglobin concentration exceeds 100 ng/μL or HA concentrations exceed 1 ng/μL, the samples could not be amplified by MBM. Inhibitors in different concentrations were amplified successfully in MDA group without any influence from inhibitors.ConclusionMDA has the capability to remove the inhibi-tion of hemoglobin and HA, which is better than MBM and has a certain value in forensic practices.
