Expression of IL-9 and its receptor mRNA in nasal mucosa of allergic rhinitis mice
10.16066/j.1672-7002.2016.09.012
- VernacularTitle:变应性鼻炎小鼠鼻黏膜白细胞介素-9及其受体mRNA表达水平的研究
- Author:
Zhaowei GU
;
He ZHAO
;
Zhiwei CAO
- Publication Type:Journal Article
- Keywords:
Rhinitis,Allergic,Perennial;
Rhinitis,Allergic,Seasonal;
Interleukin-9;
Receptors,Interleukin-9
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2016;23(9):537-540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the expression and roles of IL-9 and its receptor (IL-9R) mRNA in the nasal mucosa of ovalbumin-induced allergic rhinitis (AR) mice. METHODS Sixteen Balb/c mice were selected and randomly divided into two groups with 8 mice in each group. Mice were used for establishing the animal model of AR with ovalbumin sensitization as AR group, at the same time, the physiological saline as the control group. In each group, 4 mice were randomly taken from each group, and the pathological examination showed that the model was successful. The nasal mucosa was taken from the remaining 4 mice in each group and then to detect IL-9 and IL-9R mRNA in nasal mucosa of the two groups by using real-time quantitative PCR. RESULTS The expression of IL-9 and IL-9R mRNA could be detected in both the control and AR groups. The expression levels of IL-9 and IL-9R mRNA in the nasal mucosa of the AR group were both higher than that in the control group (P<0.05). The expression level of IL-9 mRNA was positively correlated with the expression of IL-9R mRNA in the nasal mucosa of mice (r =0.857, P<0.05). CONCLUSION IL-9 and its receptor IL-9R are involved in the development of AR, and play an important role in the development of allergic rhinitis. It provides a new perspective for the further understanding of the pathogenesis of AR and provides a new clue for the treatment of allergic rhinitis.
