Knockdown of tissue transglutaminase in SaOS-2 cell line inhibits its osteoblastic differentiation and mineralization
10.3969/j.issn.1005-4847.2016.05.002
- VernacularTitle:基因沉默组织型转谷氨酰胺酶抑制SaOS-2细胞成骨分化
- Author:
Huilei YU
;
Xiaoxue YIN
;
Zhongqiang CHEN
;
Huijie LENG
;
Chunli SONG
;
Zhongjun LIU
- Publication Type:Journal Article
- Keywords:
Tissue transglutaminase;
SaOS-2 cell line;
Osteoblastic differerntiation
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(5):448-453
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether TG2 plays an important role in the osteoblast differentiation and mineralization.Methods TG2 mRNA of SaOS-2 cells was knocked down using a lentivirus stably expressing short-hairpin ( sh) RNA targeting TG2.Then the cells were cultured in osteo-inductive medium for 14 d to measure mineralization and for 7 d to measure the levels of osteoblastic differentiation markers including ALP activity and mRNA of collagen I, osteocalcin ( OCN) and BMP-2.The wild-type SaOS-2 cells and scrambled shRNA-transducted SaOS-2 cells served as the controls. Results The controls displayed an increasing trend of the level of ALP activity and mRNA of collagen I, osteocalcin and BMP-2,and notable mineralization at 14 d.When TG2 was knocked down, ALP activity, mRNA of collagen I, osteocalcin and BMP-2 at 7d,and mineralization at 14 d were all significantly lower in comparison with the corresponding values in the controls.Conclusion TG2 is involved in the differentiation and mineralization of osteoblasts in vitro.
