Puerarin alleviates HUVEC injury induced by visfatin through up-regulating miR-155-3p
10.3969/j.issn.1005-4847.2016.05.005
- VernacularTitle:葛根素上调miR-155-3p降低内脏脂肪素诱导人脐静脉内皮细胞损伤
- Author:
Fenghua ZHOU
;
Zhiyong HUANG
;
Lei ZHANG
;
Xuegang SUN
;
Yuhua JIA
- Publication Type:Journal Article
- Keywords:
Human umbilical vein endothelial cells,UVEC;
Visfatin;
Puerarin;
miR-155-3p
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(5):465-469
- CountryChina
- Language:Chinese
-
Abstract:
Object To study the effect of puerarin on the expression of inflammatory factors and miR-155-3p in human umbilical vein endothelial cells ( HUVEC) induced by visfatin.Methods The HUVEC cell injury model was es-tablished with visfatin.Cell proliferation was measured by MTT assay.Cell apoptosis was detected by flow cytometry.The level of CRP and NF-κB was detected by ELISA, and the expression of miR-155-3p was detected by RT-PCR.The expres-sion of myeloid differentiation factor 88 ( MyD88) was identified by western blotting.Results Visfatin induced cell prolif-eration and inhibited apoptosis in HUVEC, meanwhile the expressions of both CRP and NF-κB were significantly increased, compared with that of the control group (P<0.01).Puerarin at moderate and high concentrations obviously reduced the HUVEC injury induced by visfatin, mainly through down-regulating the expression of CRP and NF-κB, as well as up-regu-lating the level of miR-155-3p in the HUVEC.MiR-155-3p mimic markedly decreased the level of MyD88, CRP and NF-κB in the HUVEC induced by visfatin (P<0.05).Conclusions Pueprarin obviously alleviates HUVEC injury induced by visfatin, probably related to down-regulating the level of MyD88, CRP, NF-κB, and up-regulating the expression of miR-155-3p in HUVEC.
