Expression of skeletal muscle glucose and energy metabolism-related genes in Guangxi Bama minipigs with type 2 diabetes mellitus

VernacularTitle:广西巴马小型猪2型糖尿病模型骨骼肌糖代谢及能量代谢相关基因的表达差异
Author: Xueyu YAN ; Qinyang JIANG ; Yanjun WU ; Jiachong LIANG ; Yafen GUO ; Ganqiu LAN
Publication Type:Journal Article
Keywords: Guangxi Bama minipig; Diabetes mellitus,type 2,T2DM; Skeletal muscle; Insulin resistance
From: Acta Laboratorium Animalis Scientia Sinica 2016;24(5):470-474
CountryChina
Language:Chinese
Abstract: Objective In this study, the glucose and energy metabolism-related genes (PGC-1α, Glut-4, ERRα, NRF-1, TFAM and mtDNA gene) were detected in type 2 diabetes mellitus ( T2DM) and non-T2DM minipigs, and the gene function was explored for T2DM pathogenesis.Methods The longissimus muscle of T2DM and non-T2DM Guangxi Bama mini-pigs was used as experiment material.The expression of glucose and energy metabolism-related genes was detec-ted by QRT-PCR.Results The expressions of PGC-1α, Glut-4, ERRαand NRF-1 genes were significantly higher than that of non-T2DM group, the expressions of TFAM and mtDNA gene were lower than that of non-T2DM group.Conclu-sions The upregulated expression of PGC-1αgene and its downstream genes Glut-4, ERRα, NRF-1 may improve the glu-cose metabolic functions in skeletal muscle in the Bama minipigs, whereas insufficient mitochondrial synthesis may induce decreasing ATP synthesis, and results in skeletal muscle insulin resistance, finally leading to the T2DM occurrence.