Once-weekly subcutaneous injection of recombinant human parathyroid hormone (1-34) promotes the bone fracture healing in rats
10.3969/j.issn.1005-4847.2016.05.008
- VernacularTitle:每周单次皮下注射rhPTH（1－34）促进大鼠的骨折愈合
- Author:
Teng MA
;
Weng ZHANG
;
Qi GUO
;
Bao HAI
;
Can LIU
;
Chunli SONG
- Publication Type:Journal Article
- Keywords:
Parathyroid hormone;
Fracture healing;
Weekly injection;
Daily injection;
Rat
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(5):481-486
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of once-weekly injection of recombinant human parathyroid hormone rhPTH(1-34) on the healing of bone fracture in rats.Methods Fifty male 3-month old SD rats were used in this study to produce unilateral tibial fracture and received internal fixation with a Kirschner needle.Based on the dose and frequency of rhPTH (1-34) injection, the rats were randomly divided into five groups (n=10 each) as follows: subcutaneous injec-tions of saline, and rhPTH in a dose of 10, 20, 10, and 20 μg/kg/d.After 4 weeks of treatment, the rats were euthana-tized and the fractured tibia were assessed by X-ray, dual energy X-ray absorptiometry ( DXA) ,micro-computed tomography ( microCT) and three-point bending test.Results The fracture healing in the 20μg/kg/w group was better than the saline group.The fracture healing in the 20μg/kg/w group was as well as the 10 μg/kg/dgroup.The BMD of 20μg/kg/w group was 26.2%higher than the saline group.The mineralized callus volume in the 20μg/kg/w group was 51.4%higher than the saline group.The total callus volume in the 20μg/kg/w group was 21.6%higher than the saline group.The ultimate load of the 20 μg/kg/w group was 29.3% higher than the saline group.There was no significant difference between the 20 μg/kg/w group and 10 μg/kg/d group in BMD, bone micro-architecture, and biomechanical strength ( P >0.05 ) . Conclusions Once weekly injection of rhPTH (1-34)can promote the bone fracture healing.
