Effects of CurTn on proliferation of VSMC
10.3969/j.issn.1001-1978.2016.11.010
- VernacularTitle:姜黄素烟酸酯对血管平滑肌细胞增殖及ERK1/2信号通路的影响
- Author:
Dongmei YANG
;
Wei YANG
;
Fei QIU
;
Siyu SUN
;
Jianxiong CHEN
;
Caiping ZHANG
;
Guozuo XIONG
;
Qinhui TUO
;
Duanfang LIAO
- Publication Type:Journal Article
- Keywords:
curcumin trinicotinate;
niacin;
curcu-min;
VSMC;
proliferation;
ERK;
mechanism
- From:
Chinese Pharmacological Bulletin
2016;32(11):1526-1530
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the anti-proliferation effects of curcumin trinicotinate ( CurTn ) on vascular smooth muscle cell ( VSMC ) and its mechanism. Methods The cells were cultured in DMEM supple-mented with 10% fetal bovine serum. MTT assay was used to examine cell proliferation. FCM was used to observe cell cycle. The expressions of PCNA, Cy-clinD1 and p-ERK1/2 were analyzed using Western blot. Results CurTn could inhibit the proliferation of VSMC and showed a certain amount-time relationship. What’ s more, CurTn could increase the G1 phase pro-portion of cell, decrease the S phase proportion and the expression level of PCNA protein. It was also found that CurTn significantly inhibit the protein expression of p-ERK1/2 and Cyclin D1 . Conclusion CurTn may inhibit the proliferation of VSMC via downregulating the expression of CyclinD1 and p-ERK1/2 .
