Effect of neferine on proliferation and invasion of human hepatocellular carcinoma cell line HepG2 and Bel-7402
10.3969/j.issn.1001-1978.2016.11.012
- VernacularTitle:甲基莲心碱体外抑制人肝癌细胞增殖和侵袭的作用机制研究
- Author:
Yajun ZHOU
;
Jing SHI
;
Geng TIAN
;
Hanxin ZHOU
;
Guoquan GAO
- Publication Type:Journal Article
- Keywords:
neferine;
human hepatocellular carcinoma cell HepG2;
human hepatocellular carcinoma cell Bel-7402;
proliferation;
invasion;
RhoA;
RhoC;
ROCK
- From:
Chinese Pharmacological Bulletin
2016;32(11):1539-1542
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of neferine on proliferation and invasion of human hepatocellular car-cinoma. Methods HepG2 and Bel-7402 cells were cultured in vitro with different concentrations of nefer-ine, then cell proliferation was observed by CCK-8 as-say; cell invasion was observed by transwell invasion assay; the protein expression of RhoA, RhoC and ROCK was detected by Western blot. Results CCK-8 results showed that neferine could significantly inhibit cell proliferation in a dose-dependent manner compared with the control group. Transwell invasion assay showed that cell invasion was significantly decreased with neferine 3 μmol · L-1 . Western blot results showed that RhoA, RhoC and ROCK protein expres-sion was decreased when neferine was co-incubated with hepatocellular carcinoma cells. Conclusion Nef-erine can inhibit proliferation and invasion of HepG2 and Bel-7402 cells, which is mediated mainly by the inhibition of RhoA, RhoC and ROCK protein expres-sion.
