Inhibitory effect of paeoniflorin on inflammatory chemokines and their receptor in a mouse model of asthma
10.3969/j.issn.1005-4847.2016.05.004
- VernacularTitle:芍药苷对哮喘模型小鼠气道炎症趋化因子及受体的干预作用
- Author:
Jiaqi LIU
;
Zhengxiao ZHAO
;
Ying WEI
;
Fei XU
;
Hongying ZHANG
;
Mihui LI
;
Linwei LU
;
Kai WEI
;
Jingcheng DONG
- Publication Type:Journal Article
- Keywords:
Bronchial asthma;
Airway inflammation;
Chemokines;
Receptor;
NF-κB;
Paeoniflorin;
Mouse
- From:
Acta Laboratorium Animalis Scientia Sinica
2016;24(5):460-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of paeoniflorin on inflammatory chemokines and their receptor in a rat model of ovalbumin-induced asthma.Methods Sixty 6-week old SPF female BALB/c mice were used in this study.To es-tablish a mouse model of asthma by sensitizing and challenging with ovalbumin.ELISA was used to analyze the serum IL-6 and TNF-α, and the specific IgE against ovalbumin ( OVA-IgE ) , CCL19 and CCL21 in bronchoalveolar lavage fluid (BALF).RT-PCR was performed to determine CCR7mRNA and protein expression of chemokine receptor CCR7, and the level of NF-κB was tested by Western blot.ResultsIn In the paeoniflorin groups, the serum IL-6 and TNF-αlevels were significantly lower, and the OVA-IgE, CCL19 and CCL21 levels in BALF were significantly reduced, compared with that in the control group.CCR7mRNA and protein expression of chemokine receptor CCR7 and NF-κB in the lung were significant-ly reduced by paeoniflorin.Conclusions Paeoniflorin has a remarkably inhibitory effect on the airway inflammatory chemo-kines CCL19/CCL21 and the receptor CCR7 in the mouse model of asthma.
