miR-26a suppresses gastric cancer cell invasion by targeting MMP16
10.19401/j.cnki.1007-3639.2016.10.002
- VernacularTitle:miR-26a靶向MMP16参与调控胃癌细胞侵袭作用研究
- Author:
Zhiyu CHEN
;
Chenchen WANG
;
Jian HU
;
Xiaoying ZHAO
;
Wenhua LI
;
Xiaodong ZHU
- Publication Type:Journal Article
- Keywords:
miR-26a;
Gastric cancer;
Matrix metalloproteinase-16;
Invasion
- From:
China Oncology
2016;26(10):813-819
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Invasion and metastasis lead to poor prognosis in gastric cancer. In this study, we investigated the potential function of miR-26a in gastric cancer.Methods:Real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR) was used to detect the expression of miR-26a in gastric cancer cells.In vitro CCK-8 assay, cloning formation assay and Matrigel-Transwell assay were used to evaluate the proliferation, migration and invasion of gastric cancer cells. A luciferase reporter assay was also conducted to confirm that matrix metallo-proteinase-16 (MMP16) is a direct target of miR-26a.Results:miR-26a was down-regulated in gastric cancer tissues compared with that in non-cancerous tissues. Functional studies showed that miR-26a inhibited cell proliferation, col-ony formation, cell motility and invasion. However, miR-26a had no effect on cell proliferation. We also characterized MMP16 as a direct target of miR-26a. We showed that knocking down MMP16 in gastric cancer cells signiifcantly de-creased MMP16 expression and inhibited cell invasion, whereas ectopic MMP16 expression signiifcantly abrogated the suppressed cell invasion induced by miR-26a.Conclusion:miR-26a suppresses gastric cancer cell invasion by targeting MMP16. miR-26a could represent a potential therapeutic target for gastric cancer.
