Optimal condition for preservation of three Achromobacter xylosoxidans bacteriophages
10.7644/j.issn.1674-9960.2016.10.009
- VernacularTitle:3株木糖氧化无色杆菌烈性噬菌体的最佳保存方法研究
- Author:
Erna LI
;
Yanyan MA
;
Weiwei CHENG
;
Aimin JIANG
;
Jing YUAN
;
Xiangna ZHAO
- Publication Type:Journal Article
- Keywords:
Achromobacter xylosoxidans;
bacteriophage;
preservation
- From:
Military Medical Sciences
2016;40(10):814-818
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find simple and effective methods of preservation for Achromobacter xylosoxidans bacteriophages.Methods The plaque forming unit( PFU) of surviving phages under different preservation conditions and temperatures at different time points was determined.Results and Conclusion The titers of phiAxp-1 and phiAxp-2 main-tained at the initial 1010 PFU/ml, and that of phiAxp-3 decreased slightly from 1011 PFU/ml to 1010 PFU/ml 16 months after the three Achromobacter xylosoxidans bacteriophages were stored in glycerol and dimethyl sulfoxide at -80℃, -20℃and 4℃.When stored in chloroform at 4℃for 16 months, the titers of all the three phages decreased slightly but were higher than at other temperatures (-80℃, -20℃, room temperature, and 37℃) .Thus these methods can effectively preserve Achromobacter xylosoxidans bacteriophages.
