Correlation of miR-92a in plasma with prognosis in osteosarcoma
10.19401/j.cnki.1007-3639.2016.08.005
- VernacularTitle:血浆miR-92a与骨肉瘤预后相关性研究
- Author:
Wei MENG
;
Li ZHANG
;
Jianwei LI
;
Min WEI
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
miR-92a;
Real-time lfuorescent quantitative polymerase chain reaction
- From:
China Oncology
2016;26(8):670-674
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Osteosarcoma is the most common primary malignant bone tumor in the children and young adults. Until now, there is no prognostic indicator with high speciifcity and high sensitivity. This study investigated the relationship between miR-92a level in palsma and clinical pathological characteristics, as well as the prognosis in osteosarcoma.Methods:The plasma from 30 healthy volunteers who underwent physical examination and from 45 cases of osteosarcoma before and after operation were collected. The miR-92a level was detected by real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR) method. According to the relative expression≥5 of miR-92a, the osteosarcoma patients were divided into 2 groups: miR-92a high expression group and low expression group.Results:The miR-92a level in palsma from osteosarcoma is signiifcantly higer than that from healthy volunteers. Before surgery, the miR-92a level was markedly higher than after surgery. There was no correlation between the miR-92a level and the gender, age, tumor location, tumor size and histological type. However, signiifcant correlation was found with Enneking grade, tumor cell necrosis rate and lung metastasis. Compared with the low-expression group, high miR-92a expression was associated with a signiifcantly shorter overall survival using Kaplan-Meier analysis (P=0.035). Conclusion:The results suggest a signiifcant relationship between miR-92a and survival ratio. miR-92a expression level may be a useful prognostic indicator in osteosarcoma.