Determination of Solubility and Apparent Oil and Water Partition Coefficient of Saponin H1
10.6039/j.issn.1001-0408.2016.03.26
- VernacularTitle:败酱皂苷H1平衡溶解度和表观油水分配系数的测定
- Author:
Qilong FANG
;
Hongwei LIU
;
Jiangyun LIU
;
Lili HAO
;
Shilin YANG
- Publication Type:Journal Article
- Keywords:
Saponin H1;
HPLC-ELSD;
Equilibrium solubility;
Apparent oil and water partition coefficient
- From:
China Pharmacy
2016;27(3):364-366
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the solubility and apparent oil and water partition coefficient (lg P) of saponin H1,and provide reference for dosage form design and druggability research of saponin. METHODS:HPLC-ELSD was conducted to deter-mine the equilibrium solubility of saponin H1 in different organic solvents and pH buffer solutions;shaking flask was applied to de-termine lg P value of saponin H1. RESULTS:The equilibrium solubility of saponin H1 in water,methanol and ethanol at 25 ℃ was 0.09175 g/L,96.51 g/L and 46.89 g/L,respectively. The solubility increased apparently at high pH within pH range at 7.6-10.0;the lg P value was between 0.695-0.773 in buffers within pH range at 6.0-8.0. CONCLUSIONS:Saponin H1 and the oleanolic acid type saponins belong to low solubility, low transmission components, so it is not suitable for use as a conventional oral formulation is developed.
