Content Determination of Hordenine in Zang Medicine Herba Aconiti by HPLC
10.6039/j.issn.1001-0408.2016.03.27
- VernacularTitle:HPLC法测定藏药榜嘎中大麦芽碱的含量
- Author:
Dashuang QING
;
Xin WANG
;
Yao QIN
;
Lincai YAO
;
Siping WANG
;
Yong YANG
;
Weizao LUO
- Publication Type:Journal Article
- Keywords:
Herba Aconiti;
HPLC;
Heteratisine;
Content determination
- From:
China Pharmacy
2016;27(3):367-369
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of hordenine in Zang medicine Herba Aconiti. METHODS:HPLC was performed on the column of Gemini-NX C18 with mobile phase of acetonitrile-0.25 mol/l Ammonium ace-tate solution(ammonia water to adjust the pH to 9.5,gradient elution,at flow rate of 1 ml/min,the detection wavelength was 230 nm,the column temperature was 30 ℃,and the injection volume was 20 μl.RESULTS:The linear range of hordenine was 3.276-819μg/ml(r=0.999 5);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 96.21%-104.04%(RSD=1.23%,n=9). CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the content determi-nation of hordenine in Zang medicine Herba Aconiti.
