Simultaneous Determination of Inulicin and Deacetylinulicin in Inulae Flos by HPLC
10.6039/j.issn.1001-0408.2016.03.28
- VernacularTitle:HPLC法测定旋覆花药材中旋覆花素和去乙酰旋覆花素的含量
- Author:
Liman MA
;
Zhen LIU
;
Mingying SHANG
;
Guangxue LIU
;
Feng XU
;
Shaoqing CAI
- Publication Type:Journal Article
- Keywords:
Inulae Flos;
Inulicin;
Deacetylinulicin;
Content determination;
HPLC
- From:
China Pharmacy
2016;27(3):369-371
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method of simultaneous determination of inulicin and deacetylinulicin in Inulae Flos. METHODS:HPLC was performed on the column of Zorbax SB-C18 with mobile phase of acetonitrile-water(gradient elution)at a flow rate of 1.0 ml/min,the column temperature was 25 ℃,the detection wavelength was 210 nm,and the injection volume was 10 μl. RESULTS:The linear range was 0.000 2-0.005 μg/ml(r=0.999 8)for inulicin and 0.000 1-0.001 7 μg/ml(r=0.999 4)for deacetylinulicin;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recoveries were 99.63%-103.56%(RSD=1.26%,n=9)and 95.98%-101.21%(RSD=1.84%,n=9),respectively. CONCLUSIONS:The method is simple,accurate and reliable,and can be used for the quality evaluation of Inulae Flos.
