Simultaneous Determination of Geniposide,Berberine Hydrochloride and Baicalin in Annao Pill by HPLC
10.6039/j.issn.1001-0408.2016.03.35
- VernacularTitle:HPLC法同时测定安脑丸中栀子苷、盐酸小檗碱和黄芩苷的含量
- Author:
Yantao SUN
- Publication Type:Journal Article
- Keywords:
Annao pill;
Geniposide;
Berberine hydrochloride;
Baicalin;
HPLC
- From:
China Pharmacy
2016;27(3):389-391
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of geniposide,berberine hydrochloride and ba-icalin in Annao pill. METHODS:HPLC was performed on the column of Inertsil ODS-3 C18 with mobile phase of acetonitrile-0.2% phosphoric acid solution (gradient elution) at a flow rate of 1.0 ml/min,the column temperature was 30 ℃,the detection wavelength was 230 nm,and the injection volume was 10 μl. RESULTS:The linear range was 0. 041 0-0.820 0 μg for geniposide (r=0.999 9),0.037 6-0.752 0μg for berberine hydrochloride(r=0.999 9)and 0.095 2-1.904 0μg for baicalin(r=0.999 9),respec-tively;recoveries were 97.31%-100.41%(RSD=1.10%,n=6),98.32%-101.76%(RSD=1.20%,n=6) and 99.16%-101.30%(RSD=0.76%,n=6),respectively. CONCLUSIONS:The method is simple and accurate,and can be used for the quality control of Annao pill.
