Improvement of Content Determination for Polygala XanthoneⅢin Polygalae Radix
10.6039/j.issn.1001-0408.2016.03.38
- VernacularTitle:远志中远志酮Ⅲ含量测定方法的改进
- Author:
Jingjing CHEN
;
Xiang WANG
;
Yunlan LIAN
;
Yan NI
- Publication Type:Journal Article
- Keywords:
Polygalae Radix;
Polygala xanthoneⅢ;
Improved method
- From:
China Pharmacy
2016;27(3):397-399
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the content determination for polygala xanthone Ⅲ in Polygalae Radix contained in Chi-nese Pharmacopoeia (2010 edition). METHODS:HPLC was performed on the column of Hypersil BDS C18 with mobile phase of acetonitrile-0.05% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min;detection wavelength was 320 nm,column tem-perature was 25℃,and the injection volume was 10μl. RESULTS:The linear range of polygala xanthoneⅢwas 0.029-0.928 μg/ml (r=0.999 1);RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 94.66%-100.90%(RSD=2.46%,n=6). CONCLUSIONS:The method is simple,stable and reproducible. Although the determination time is prolonged,it has improved the accuracy and it is more suitable for the content determination of polygala xanthoneⅢin Polygalae Radix.
