Determination of Cholic Acid,Hyodeoxycholic Acid and Deoxycholate Acid in Naoxueshuan Tablet by HPLC-ELSD

VernacularTitle:HPLC-ELSD法同时测定脑血栓片中胆酸、猪去氧胆酸和去氧胆酸的含量
Author: Jun ZHOU ; Jie WANG ; Lei ZHANG
Publication Type:Journal Article
Keywords: HPLC-ELSD; Naoxueshuan tablet; Cholic acid; Hyodeoxycholic acid; Deoxycholate acid
From: China Pharmacy 2016;27(3):404-406
CountryChina
Language:Chinese
Abstract: OBJECTIVE:To establish a method for the contents determination of cholic acid,hyodeoxycholic acid and deoxy-cholate acid in Naoxueshuan tablet. METHODS:HPLC-ELSD was performed on the column of SHIMADZU VP-ODS C18 with mo-bile phase of acetonitrile-0.2% formic acid(gradient elution)column temperature was 30 ℃,at flow rate of 1.0 ml/min,and the vol-ume injection was 10 μl. RESULTS:The linear range was 0.039 84-0.398 4 mg for cholic acid,0.032 064-0.320 64 mg for hyode-oxycholic acid and 0.0339 52-0.339 52 μg for deoxycholate acid(r≥0.999 0),and the logarithm value of volume injection and peak area showed good linear relationship;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 101.33%-104.05%(RSD=0.95%,n=6),95.56%-101.38%(RSD=2.54%,n=6)and 96.07%-97.12%(RSD=0.44%,n=6),re-spectively. CONCLUSIONS:The method is simple and accurate,and suitable for the contents determination of cholic acid,hyode-oxycholic acid and deoxycholate acid in Naoxueshuan tablet.