Content Determination of Vincristine Sulfate for Injection by HPLC
10.6039/j.issn.1001-0408.2016.03.42
- VernacularTitle:HPLC法测定注射用硫酸长春新碱的含量
- Author:
Rong ZHU
- Publication Type:Journal Article
- Keywords:
HPLC;
Vincristine sulfate for injection;
Content
- From:
China Pharmacy
2016;27(3):407-409
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of Vincristine sulfate for injection. METHODS:HPLC was performed on the column of Agilent HC-C8 with mobile phase of 1.67% Diethylamine aqueous solution(adjusted to pH 7.5 with phosphoric acid)-methanol(38:62,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 297 nm,the column temperature was 35℃,and the injection volume was 10μl. RESULTS:The linear range of vincristine sulfate was 0.032-3.2 mg/ml (r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 99.3%-101.7%(RSD=0.6%,n=9). CONCLUSIONS:The method is accurate,reliable and sensitive,and can be used for the content determination of Vincristine sulfate for injection.
