Study on Contents Determination of Phospholipid andγ-linolenic Acid in Compound Linolenic Acid Soft Cap-sule
10.6039/j.issn.1001-0408.2016.03.44
- VernacularTitle:复方亚麻酸软胶囊中大豆卵磷脂和γ-亚麻酸含量测定的方法学研究
- Author:
Guoqi SUI
;
Guangzhi CAI
;
Liankun LI
;
Jiyu GONG
- Publication Type:Journal Article
- Keywords:
Compound linolenic acid soft capsule;
Gas chromatography;
HPLC;
Phospholipid;
γ-linolenic acid
- From:
China Pharmacy
2016;27(3):412-414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule. METHODS:HPLC was conducted to determine the content of soybean lecithin;the column was Agilent TC-C18 with mobile phase of methanol-water(84:16,V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 219 nm,the column temperature was 30 ℃,and the injection volume was 10 μl.Gas chromatography was conducted to determine the content of γ-linolenic acid in the preparation;the column was DB WAX,injector temperature was 210 ℃ by temperature pro-grammed,carrier gas was helium at a flow rate of 1 ml/min by split injection(split ratio of 1:30),and the injection volume was 0.02μl. RESULTS:The linear range was 4.64-16.24μg/ml for phospholipid(r=0.999 6)and 0.093 44-0.327 04μg/ml forγ-linole-nic acid methy ester(r=0.999 6);RSDs of precision,stability and reproducibility tests were lower than 3%;recoveries were 97.44%-99.36%(RSD=0.93%,n=6) and 97.22%-99.07%(RSD=1.01%,n=6),respectively. CONCLUSIONS:The method is simple,stable with good separation,and can be use for the contents determination of phospholipid and γ-linolenic acid in Com-pound linolenic acid soft capsule.
