Content Determination of Psoralen in Psoralea-pericarpium Juglates Liniment by HPLC
10.6039/j.issn.1001-0408.2016.06.27
- VernacularTitle:HPLC法测定补骨脂-青龙衣酊中补骨脂素的含量
- Author:
Ling TANG
;
Ruiting ZHAO
- Publication Type:Journal Article
- Keywords:
HPLC;
Psoralea;
Pericarpium juglates;
Psoralen
- From:
China Pharmacy
2016;(6):805-807
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of psoralen in Psoralea-pericarpium juglates lini-ment. METHODS:HPLC was performed on the column of Shim-pack VP-ODS C18 with mobile phase of methanol-water(60∶40, V/V)at a flow rate of 0.8 ml/min,detection wavelength was 246 nm,column temperature was 25 ℃,and injection volume was 20μl. RESULTS:The linear range of psoralen was 2.49-39.90 μg/ml;RSDs of precision,stability and reproducibility tests were lower than 2.00%;recovery was 97.23%-100.58%(RSD=1.17%,n=6). CONCLUSIONS:The method is fast,simple,accurate and re-liable,and can be used for the content determination of psoralen in Psoralea-pericarpium juglates liniment.
