Content Determination of Total Flavonoids in Morus alba
10.6039/j.issn.1001-0408.2016.06.40
- VernacularTitle:桑叶药材中总黄酮含量的测定
- Author:
Dan LYU
;
Haibo WANG
;
Xue DI
;
Zhenqiu ZHANG
- Publication Type:Journal Article
- Keywords:
Morus alba;
Total flavonoids;
UV-visible spectrophometry
- From:
China Pharmacy
2016;(6):844-845
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of total flavonoids in Morus alba. METHODS:UV-visible spectrophometry was performed with Al(NO2)3-NaNO2-NaOH color-test at the wavelength of 510 nm with the reference of rutin. RESULTS:The linear range of rutin was 0.031 2-0.156 mg/ml(r=0.999 9);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 95.7%-101.0%(RSD=2.1%,n=6). CONCLUSIONS:The method is simple,sta-ble and reproducible,and can be used for the content determination of total flavonoids in M. alba.
