Determination of Valsartan in Human Plasma and Urine by LC-MS/MS and Its Pharmacokinetic Study
10.6039/j.issn.1001-0408.2016.05.13
- VernacularTitle:LC-MS/MS法测定人血浆及尿液中缬沙坦的浓度及其药动学研究
- Author:
Shiyin FENG
;
Xiaolan YONG
;
Juan HUANG
;
Xiaolin DU
;
Nan LI
;
Lantian WANG
- Publication Type:Journal Article
- Keywords:
LC-MS/MS;
Valsartan;
Plasma concentration;
Urine concentration;
Pharmacokinetics
- From:
China Pharmacy
2016;27(5):619-621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop a method for the determination of valsartan concentration in human plasma and urine. METHODS:Plasma sample were acidified and extracted with diethyl ether for analysis,and urine sample was diluted directly for analysis. The samples were all determined by LC-MS/MS,and the separation was performed on a Aglient ZORBAX SB-C18 column with mobile phase consisted of acetonitrile and 0.1% formic acid (gradient elution) at flow rate of 0.2 ml/min. Ion transition was determined ESI ion source under multiple ion reaction monitoring with quantitative pair m/z 436.4→253.2 and qualitative ion pair m/z 436.4→291.3 for valsartan,and quantitative pair m/z 423.4→207.1 and m/z 423.4→180.2 for internal standard losartan. RE-SULTS:The linear range of valsartan were 4-5 000 ng/ml in plasma and 20-50 000 ng/ml in urine;the limit of quantification were 4 ng/ml and 20 ng/ml;plasma extraction recovery of valsartan were 61.21%-70.30%. The variation coefficient of internal standard normalized matrix effect were 3.20% and 11.21%. The within-day and between-day RSDs were no more than 8.34%. CONCLU-SIONS:The method is proved to be rapid and sensitive,and suitable for the determination of valsartan in human plasma and urine and pharmacokinetics study.
