Content Determination of L-epicatechin in Actinidia arguta by HPLC
10.6039/j.issn.1001-0408.2016.03.31
- VernacularTitle:HPLC法测定藤梨根中表儿茶素的含量
- Author:
Jun HE
;
Bingzhi MA
;
Yingying LIANG
;
Xiaoxue WANG
;
Xiao LIU
- Publication Type:Journal Article
- Keywords:
HPLC;
Actinidia arguta;
L-epicatechin;
Content determination
- From:
China Pharmacy
2016;27(3):378-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of L-epicatechin in Actinidiae arguta. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.2%Acetic acid solution(15:85,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 25 ℃,and the volume injection was 10 μl. RESULTS:The linear range of L-epicatechin was 10.47-167.52 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2%;average recovery was 98.07%-101.71%(RSD=1.39%,n=6). CONCLUSIONS:The method is simple, accurate and reliable,and suitable for the content determination of L-epicatechin in A. arguta.
