Content Determination of Carbohydrates in Osseous and Cucumis Extract Injection by Ion Chromatography
10.6039/j.issn.1001-0408.2016.03.34
- VernacularTitle:离子色谱法测定骨瓜提取物注射液中糖的含量
- Author:
Wei ZHANG
;
Tong ZHANG
;
Lianjie REN
;
Wen LYU
;
Chunxia HAN
;
Changming ZHOU
- Publication Type:Journal Article
- Keywords:
Ion chromatography;
Osseous and cucumis extraction injection;
Carbohydrates
- From:
China Pharmacy
2016;27(3):386-389
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of carbohydrates in Osseous and cucumis extract injection. METHODS:Ion chromatography was conducted on the column of Dionex CarboPac PA20 with mobile phase of water-1 mol/L Sodium acetate solution-250 mmol/L Sodium hydroxide solution(gradient elution)at flow rate of 0.45 ml/min,column tem-perature was 35 ℃,and injection volume was 10 μl,detector was electrochemical analysis detector. RESULTS:The linear ranges of galactose,glucose,mannose,sucrose and fructose were 0.5-10.0μg/ml(r>0.998 0);RSDs of precision,stability and reproduc-ibility tests were lower than 2%;recovery was 94.8%-99.1%(RSD=0.52%-0.89%,n=9). CONCLUSIONS:The method is sim-ple,fast and accurate,and can be used for the determination of carbohydrates in Osseous and cucumis extract injection.
