Study on the Quality Standard for Chaige Ganmao Mixture
10.6039/j.issn.1001-0408.2016.03.36
- VernacularTitle:柴葛感冒合剂的质量标准研究
- Author:
Qianqian CHEN
;
Hongsheng SUN
- Publication Type:Journal Article
- Keywords:
Chaige ganmao mixture;
Quality standard;
TLC;
HPLC;
Scutellaria baicalensis;
Paeonia lactiflora;
Puerarin
- From:
China Pharmacy
2016;27(3):392-394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Chaige ganmao mixture. METHODS:TLC was conducted to iden-tify the Scutellaria baicalensis and Paeonia lactiflora;HPLC was conducted to determine the content of puerarin. The column was Zorbax Eclipse XDB-C18 with mobile phase of methanol-water(25:75,V/V)at flow rate of 1.0 ml/min,detection wavelength was 250 nm,and column temperature was 25℃,and the injection volume was 5μl. RESULTS:TLC showed clear spots and good sepa-ration. The linear range of puerarin was 18-198 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were low-er than 2%,recovery was 99.04%-99.75%(RSD=0.28%,n=6). CONCLUSIONS:The standard can be used for the quality con-trol of Chaige ganmao mixture.
