Simultaneous Determination of Puerarin,Berberine Hydrochloride and Baicalin in Erxieting Syrup by HPLC
10.6039/j.issn.1001-0408.2016.03.37
- VernacularTitle:HPLC法同时测定儿泻停糖浆中黄芩苷、盐酸小檗碱、葛根素的含量
- Author:
Yue ZHOU
;
Jianwei CHEN
- Publication Type:Journal Article
- Keywords:
Erxieting syrup;
Puerarin;
Berberine hydrochloride;
Baicalin;
HPLC
- From:
China Pharmacy
2016;27(3):394-396
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of puerarin,berberine hydrochloride and ba-icalin in Erxieting syrup. METHODS:HPLC was performed on the column of Kromasil-C18 with mobile phase of methanol-0.2%Phosphoric acid solution (gradient elution) at flow rate of 1.0 ml/min,column temperature was 30 ℃,detection wavelength was 275 nm,and the volume injection was 10 μl. RESULTS:The linear was 10.43-83.44 μg/ml for puerarin,10.71-85.68 μg/ml for ber-berine hydrochloride and 12.58-100.64 μg/ml for baicalin(r≥0.999 3);RSDs of precision,stability and reproduciblity tests were lower than 2%;recoveries were 98.25%-100.87%(RSD=0.92%,n=6),99.41%-101.40%(RSD=0.68%,n=6)and 98.13%-99.76%(RSD=0.57%,n=6),respectively. CONCLUSIONS:The method is simple,reliable,can be used for the quality control of Erxiet-ing syrup.
