Study on Inhibitory Effect of Lycianthes biflora Polysaccharide on the Proliferation of Hepatic Stellate HSCs-T6 Cells in Rats and Its Mechanism
10.6039/j.issn.1001-0408.2016.04.17
- VernacularTitle:红丝线多糖对鼠源肝星状HSCs-T6细胞增殖的抑制作用及其机制研究
- Author:
Silu HE
;
Xiaobao GUO
;
Lingyuan XU
;
Aijun JIAO
- Publication Type:Journal Article
- Keywords:
Lycianthes biflora polysaccharide;
Hepatic stellate HSCs-T6 cells;
Inhibitory rate of proliferation;
Collagen;
TGF-βpathway
- From:
China Pharmacy
2016;(4):485-487
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study inhibitory effect of Lycianthes biflora polysaccharide on the proliferation of hepatic stellate HSCs-T6 cells in rats and its mechanism. METHODS:After treated with 0(blank control),50,100,200μg/ml L. biflora polysac-charide for 24 and 48 h,the activity of hepatic stellate HSCs-T6 cells in rats was determined by MTT assay and inhibitory rate of cell proliferation was calculated;the content of Hyp in supernatant was detected by immunohistochemical assay. After 48 h,the ex-pression of cellular α-smooth muscle actin(α-SMA)was measured by immunohistochemical assay;both transforming growth fac-tor β1(TGF-β1)and Smad3 were measured by Western blot assay. RESULTS:Compared to blank control,50,100 and 200 μg/ml L. biflora polysaccharide could inhibit the proliferation of HSCs-T6 cells;cell inhibitory rates were 39.84%-69.31% and 45.16%-82.93% respectively after treated for 24 and 48 h,which were positively associated with time and concentration. The con-tents of Hyp in supernatant were 178.36-93.25 μg/ml and 131.94-68.74 μg/ml respectively after treated with different concentrations of PRP for 24 and 48 h,which were negatively associated with time and concentration. The protein level of TGF-β1 and Smad3 de-creased after treated with L. biflora polysaccharide for 48 h(P<0.01). CONCLUSIONS:L. biflora polysaccharide can inhibit the proliferation of hepatic stellate HSCs-T6 cells in rats,and its mechanism is associated with the inactivation of endogenous TGF-βpathway for reducing collagen production.
