Optimization of the Content Determination Conditions of Total Alkaloids from Zhuang Medicine Munronia delavayi and Comparison of the Contents in M. delavayi from Different Producing Areas
10.6039/j.issn.1001-0408.2016.04.14
- VernacularTitle:壮药矮陀陀中总生物碱含量测定条件优选及不同产地药材含量比较
- Author:
Wenfang MA
;
Yurong TANG
;
Pinghua YAN
;
Xiangyan ZENG
;
Yi CAI
- Publication Type:Journal Article
- Keywords:
Munronia delavayi;
Total alkaloids;
Content determination;
Orthogonal test
- From:
China Pharmacy
2016;(4):476-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To optimize the content determination conditions of total alkaloids from Zhuang medicine Munronia delavayi,and to determine the content of total alkaloids in M. delavayi from different producing areas. METHODS:With the con-tent of total alkaloids as index,using solvent amount,ultrasonic time,ultrasonic extraction times and pH value of buffer as fac-tors,the content determination conditions of total alkaloids from Zhuang medicine M. delavayi were optimized by orthogonal test. Optimized content determination conditions were adopted to determine the content of total alkaloids in M. delavayi from 18 produc-ing areas in different harvest time. RESULTS:The optimum content determination conditions were as follows as the amount of sol-vent(CHCl3)20 ml,ultrasonic processing for 3 times,lasting for 15 min each time,pH value of buffer 4.5. The contents of total alkaloids in M. delavayi from 18 producing areas were between 0.6-11.98 mg/g,showing great difference. M. delavayi from Long-lin county and Tianlin county harvested in Oct. had the lowest content of total alkaloids. CONCLUSIONS:Optimized content deter-mination condition can be used for the content determination of total alkaloids in Zhuang medicine M. delavayi and quality control of it. The content determination of total alkaloids in M. delavayi is related to producing area and harvest time.
