Effects of Baicalin on Human Osteosarcoma MG63 Cells Apoptosis and the Expression of MMPs
10.6039/j.issn.1001-0408.2016.04.18
- VernacularTitle:黄芩苷对人骨肉瘤MG63细胞凋亡及MMPs表达的影响
- Author:
Zhihua SHEN
;
Jingjing GUO
- Publication Type:Journal Article
- Keywords:
Baicalin;
Human osteosarcoma MG63 cells;
Matrix metalloproteinase;
Cell apoptosis
- From:
China Pharmacy
2016;(4):488-490
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of baicalin on human osteosarcoma MG63 cells apoptosis and the expression of MMPs. METHODS:Treated with 0(blank control),5,10,20,40,80,160,320 μg/ml baicalin for 24 h,the survival rate,the expression amount of MMP-2 and MMP-9 were detected,and IC50 was calculated. Cell apoptosis was observed. RESULTS:Com-pared with blank control group,after treated with baicalin,survival rate of MG63 cells decreased,while apoptotic amount in-creased,the expression amount of MMP-2 and MMP-9 decreased;there was statistical significance in the expression amount de-crease of MMP-2 and MMP-9 in MG63 cells after treated with 320 μg/ml baicalin(P<0.05);IC50 was(40.21±9.20)μg/ml,all responses were in concentration-dependent manner. CONCLUSIONS:Baicalin can inhibit the proliferation of MG63 cells,induce cell apoptosis,and inhibit the expression of MMP-2 and MMP-9 in cells under high concentration.
