Inhibitory Effects of Propofol on PC12 Cells Injury Induced by Glutamic Acid via MAPK/ERK Signal Pathway
10.6039/j.issn.1001-0408.2016.01.20
- VernacularTitle:丙泊酚通过MAPK/ERK信号通路对谷氨酸诱导的神经PC12细胞损伤的抑制作用
- Author:
Zheng LI
;
Xiangyu GAO
;
Zhe LIU
;
Nan LIANG
;
Zheng NAN
- Publication Type:Journal Article
- Keywords:
Propofol;
PC12 cells;
Apoptosis;
Mitogen-activated protein kinase/extra-cellular regulated kinase signal pathway
- From:
China Pharmacy
2016;27(1):61-63
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study inhibitory effects of propofol on PC12 cells injury induced by glutamic acid via mitogen-acti-vated protein kinase/extra-cellular regulated kinase (MAPK/ERK) signal pathway. METHODS:PC12 cells were randomized into normal control group,model group(10 mmol/L glutamic acid),propofol low-concentrations,medium-concentrations and high-con-centrations groups(12.5,25,50 μmol/L+10 mmol/L glutamic acid). The optical density of cells,cell apoptosis,the phosphoryla-tion of ERK1/2 and the expression of c-fos,Bax,Bcl-2 were detected after treated with relevant medicine for 48 h. RESULTS:Compared with normal control group,optical density of cells,the phosphorylation of ERK1/2 and Bcl-2 decreased in model group (P<0.01);apoptotic rate,the expression of c-fos and Bax increased (P<0.01). Compared with model group,optical density of cells,the expression of Bcl-2 and the phosphorylation of ERK1/2 increased in propofol group (P<0.01);apoptosis rate,the ex-pression of c-fos and Bax decreased (P<0.05 or P<0.01). There were statistical significant between the different concentrations (P<0.01). CONCLUSIONS:Propofol can inhibit the apoptosis of PC12 cells induced by glutamic acid,which is associated with the up-regulation of ERK1/2 phosphorylation.
