Preparation and Tumor Inhibition Effect of Transferrin Modified Paclitaxel-loaded Liposome
10.6039/j.issn.1001-0408.2016.01.14
- VernacularTitle:转铁蛋白修饰紫杉醇脂质体的制备及其抑瘤作用
- Author:
Cailing JIN
;
Shupeng ZHAO
;
Min ZHANG
;
Ying WANG
;
Xiaoge KOU
;
Ping LU
- Publication Type:Journal Article
- Keywords:
Transferrin;
Paclitaxel;
Liposome;
Lung cancer HepG2 cell;
Nude mice;
Proliferation inhibition rate;
Tumor inhi-bition rate
- From:
China Pharmacy
2016;27(1):44-47
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To prepare transferrin modified paclitaxel-loaded liposome(TF-PTX-LP),and to study the tumor in-hibition effect. METHODS:TF-PTX-LP was prepared by thin-film method,and morphology of TF-PTX-LP was observed. Qualita-tive and quantitative investigation were used to value the uptake efficiency of TF-LP and LP by HepG2 cells. The proliferation inhi-bition rate of HepG2 cells was investigated after treated with PTX,PTX-LP and TF-PTX-LP for 24,48 and 72 h. Tumor spheres were prepared by using HepG2 cells. Effects of normal saline,PTX,PTX-LP and TF-PTX-LP on the volume of tumor spheres were investigated after 0,1,2,4,5,6 and 7 d treatment. HepG2 tumor-bearing nude mice model was induced. Inhibitory effects of normal saline,PTX,PTX-LP and TF-PTX-LP(8.5 mg/kg by PTX)on transplantable tumor of tumor-bearing nude mice were in-vestigated. RESULTS:TF-PTX-LP showed uniform spherical shape,with particle size of 100-120 nm. The fluorescence intensity of HepG2 cells treated with TF-LP was stronger than that treated with LP(P<0.01). Compared with PTX and PTX-LP,TF-PTX-LP showed higher proliferation inhibition rate(P<0.01). Compared with normal saline,PTX and PTX-LP,tumor spheres were small-er in volume after treated with TF-PTX-LP,and inhibition rate of tumor was higher in tumor-bearing nude mice;there were statisti-cal significance after treated for 6,7 d(P<0.01). The proliferation inhibition rate and tumor spheres volume changed in time-de-pendent manner. CONCLUSIONS:TF-PTX-LP which owns good tumor inhibition effect is prepared successfully.
