Effects of Lactobacillus fermentum on the Inhibition of Cytoxan on Human Colorectal Cancer HCT116 Cells
10.6039/j.issn.1001-0408.2016.01.15
- VernacularTitle:特异乳酸菌对环磷酰胺抗人大肠癌HCT116细胞作用的影响
- Author:
Huaqing LIU
;
Rui LIN
;
Yong CHEN
;
Rui LIU
;
Hui HUANG
;
Xiaoling LUO
- Publication Type:Journal Article
- Keywords:
Lactobacillus fermentum;
Cytoxan;
Human colorectal cancer HCT116 cell;
Nude mice;
Survival rate;
Inhibition rate
- From:
China Pharmacy
2016;27(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Lactobacillus fermentum(Lb-f)on inhibition of cytoxan(CTX) on HCT116 cells of human colorectal cancer in vitro and in vivo. METHODS:HCT116 cells were cultured in vitro. MTT assay was used to in-vestigate the effects of 4,2,1μg/ml CTX and combined with 10μg/ml Lb-f on the survival rate of HCT116 cells. HCT116 cell xe-nograft tumor nude mice model was induced to investigate the effects of 100,50,25 mg/kg CTX and combined with 180 mg/kg Lb-f on tumor volume,tumor weight,relative tumor inhibition rate,the sensitization effect of chemotherapy (by q value),the number of peripheral blood leucocyte and platelet. RESULTS:Compared with CTX alone,CTX combined with Lb-f had no signifi-cant effect on survival rate of HCT116,relative inhibition rate of tumor volume in nude mice, relative inhibition rate of tumor weight and q value (P>0.05),but increased the number of peripheral blood leucocyte and platelet (P<0.05). CONCLUSIONS:No synergistic effects of Lb-f is found on the inhibition of CTX on the growth of HCT116 in vitro and in vivo;Lb-f can inhibit the decrease of peripheral blood leucocyte and platelet of HCT116 cell xenograft tumor nude mice induced by CTX.
