Study on the Quality Standard for Yiwei Granule
10.6039/j.issn.1001-0408.2015.36.29
- VernacularTitle:益胃颗粒的质量标准研究
- Author:
Yanqin CHENG
;
Xu LIU
;
Liyan ZHAO
;
Mingchun LI
- Publication Type:Journal Article
- Keywords:
Yiwei granule;
Paeoniflorin;
TLC;
HPLC;
Content determination
- From:
China Pharmacy
2015;26(36):5123-5125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard for Yiwei granule. METHODS:TLC was conducted for the qualitative identification of Coptis chinensis,Corydalis yanhusuo and Schisandra chinensis;HPLC was conduced for the content determination of paeoniflorin. The column was XDB C18 with mobile phase of methanol-water(25:75,V/V)at flow rate of 1.0 ml/min,detection wave-length was 230 nm and injection volume was 20μl. RESULTS:The TLC showed clear spots and good separation. The linear range of paeoniflorin was 7.575-60.6 μg/ml(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 2.0%,recov-ery was 96.97%-102.51%(RSD=1.92%,n=9). CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Yiwei granule.
