Content Determination of Dextran70 in Compound Dextran70 Eye Drop by HPLC-RID
10.6039/j.issn.1001-0408.2015.36.30
- VernacularTitle:HPLC-RID法测定复方右旋糖酐70滴眼液中右旋糖酐70的含量
- Author:
Li WANG
;
Ruizhong GONG
;
Zetao LIU
- Publication Type:Journal Article
- Keywords:
HPLC-RID;
Compound dextran70 eye drop;
Dextran70;
Content determination
- From:
China Pharmacy
2015;26(36):5126-5128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for determination of dextran70 in Compound dextran70 eye drop. METHODS:HPLC-RID was performed on the column of Phenomenex PolySep-GFC-P 4000 with the mobile phase of 0.7% Na2SO4 solution. The flow rate was 0.5 ml/min,column temperature was 35 ℃ and injection volume was 50 μl,and the inner temperature of refrac-tive index detector was 40 ℃. RESULTS:The linear range of dextran70 was 0.1-5.0 mg/ml(r=0.999 5);RSDs of precision,sta-bility and reproducibility tests were lower than 2%;average recovery was 97.45%-101.76%(RSD=0.57%,n=9). CONCLU-SIONS:The method is simple,fast and reliable with high separation and short analysis time,and can be used for the content deter-mination of dextran70 in Compound dextran70 eye drop.
