Study on the HPLC Fingerprints of Dangua Yangmu Cream
10.6039/j.issn.1001-0408.2015.36.31
- VernacularTitle:丹瓜养目膏的HPLC指纹图谱研究
- Author:
Yuhui QIN
;
Yuehui LI
;
Dianbo TAN
;
Yanjun LING
;
Yin WANG
;
Yu PENG
- Publication Type:Journal Article
- Keywords:
Dangua yangmu cream;
Fingerprint;
HPLC;
Aurantio-obtusin
- From:
China Pharmacy
2015;26(36):5128-5130
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the HPLC fingerprints for Dangua yangmu cream. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.026% phosphoric(gradient elution)at flow rate of 0.8 ml/min,the detection wavelength was 270 nm,the column temperature was 25 ℃ and the injection volume was 20 μl. The chromatographic peak of aurantio-obtusin was used as reference peak to determine the 10 batches of Dangua yangmu cream,and Similarity Evalua-tion System for Chromatographic Fingerprint of TCM(version 2.0)was conducted to identify common peaks and evaluate similari-ty. RESULTS:There were totally 25 common peaks in the 10 batches of Dangua yangmu cream,and the similarity was not lower than 0.921. The validation results showed the fingerprints of 10 batches of Dangua yangmu cream had good consistency with the ref-erence fingerprints. COMCLUSIONS:The established method is specific and reliable,and can provide basis for the quality evalua-tion and control of Dangua yangmu cream.
