Content Determination of Astragaloside in Danggui Buxue Formula Granule by HPLC
10.6039/j.issn.1001-0408.2015.36.32
- VernacularTitle:HPLC法测定当归补血配方颗粒中黄芪甲苷的含量
- Author:
Guangsheng LI
;
Fang QIN
;
Jiling YANG
;
Wenwen HU
;
Junli ZHANG
;
Weixin ZHANG
- Publication Type:Journal Article
- Keywords:
Danggui buxue formula granule;
Astragaloside;
Content determination;
HPLC;
Melting condition
- From:
China Pharmacy
2015;26(36):5131-5133
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of astragaloside in Danggui buxue formula granule, and investigate the influence of different conditions of dissolution on the content. METHODS:HPLC was performed on the column of Aglient C18 with mobile phase of acetonitrile-water(28:72,V/V)at flow rate of 1.0 ml/min,column temperature was 25 ℃,and the injection volume was 10 μl. RESUITS:The linear range of astragaloside was 2.91-17.46 μg;RSDs of precision,stability were lower than 2%,and reproducibility tests was 3.63%,recovery was 95.76%-98.20%(RSD=1.03%,n=6). The optimized use method was mixing the two granules with 80 ℃ hot water and keeping heat preservation for 5 min successively before taken. CON-CLUSIONS:The method is simple and reproducible,and can be used for the content determination of astragaloside in Danggui bux-ue formula granule.
