Research on the Method for the Limit Detection of Aconitine in Wuqi Shujin Tongluo Tablet
10.6039/j.issn.1001-0408.2015.36.37
- VernacularTitle:乌芪舒筋通络片中乌头碱限量检测方法研究
- Author:
Zhenyao CHEN
;
Jinying CHEN
;
Weisheng YAO
- Publication Type:Journal Article
- Keywords:
Wuqi shujin tongluo tablet;
Aconitine;
Limit;
TLC
- From:
China Pharmacy
2015;26(36):5144-5146
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the limit detection of aconitine in Wuqi shujin tongluo tablet. METHODS:TLC was conducted to identify the aconitine;TLC plate was silica gel G plate,developing solvent was toluene-ethyl acetate-chloro-form-acetone-ammonia(20:18:3:6:1,V/V/V/V/V),chromogenic agent was bismuth potassium iodide test solution and sodium ni-trite ethanol test solution;and durability investigation and detection limit detection were used to optimize the TLC plate,tempera-ture and humidity. RESULTS:TLC showed the aconitine had clear spots and negative control without interference. The durability was good;detection limit was 0.9 μg;available TLC plate was Merck HPTLC prefabricated plate, silica gel G TLC plate setf-made silica gel G TLC plate with adhesive of sodium carboxymethycellulose;temperature was 5-16 ℃ and humidity was 32%-72%. CONCLUSIONS:The method is simple and reproducibility,and can be used for the limit detection of aconitine in Wuqi shujin tongluo tablet.
