Content Determination of L-shikonin in Zilian Liniment and Evaluation the Uncertainty of 2 Calculation Methods
10.6039/j.issn.1001-0408.2015.36.39
- VernacularTitle:HPLC法测定紫连搽剂中左旋紫草素的含量及两种不同计算方法的不确定度评价
- Author:
Hongmei ZHANG
;
Xiao JIANG
;
Xueqi LI
- Publication Type:Journal Article
- Keywords:
L-shikonin;
Zilian liniment;
Content determination;
Uncertainty
- From:
China Pharmacy
2015;26(36):5149-5151
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of L-shikonin in Zilian liniment and evaluate the uncertainty by 2 calculation methods. METHODS:HPLC was performed on the column of Waters C18 with mobile phase of metha-nol-0.025 mol/L phosphate(85:15,V/V)at flow rate of 1.0 ml/min,the detection wavelength was 516 nm,column temperature was room temperature,and the injection volume was 10 μl. Quantitative analysis was conducted for the process and procedures calculat-ed by standard curve method and external standard method,and the uncertainty was evaluated. RESULTS:The linear range of L-shi-konin was 4.024-80.48μg/ml;RSDs of precision,stability and reproducibility test were lower than 2.0%;recovery was 88.5%-93.1%(RSD=2.0%,n=6). Standard curve method was used to determine the content,and the expanded uncertainty was 0.70 μg/ml;ex-ternal standard method was used to determine the content,and the expanded uncertainty was 0.30 μg/ml. CONCLUSIONS:Stan-dard curve method is more economical,more convenient and lower uncertainty in the content determination of L-shikonin in Zilian liniment.
