Content Determination of Paeoniflorin in Bailing Tablet by HPLC and Its Uncertainty Evaluation
10.6039/j.issn.1001-0408.2015.36.40
- VernacularTitle:HPLC法测定白灵片中芍药苷的含量及不确定度评价
- Author:
Jianming LI
;
Huichan HOU
;
Lihong GU
- Publication Type:Journal Article
- Keywords:
Bailing tablet;
Paeoniflorin;
Content determination;
Uncertainty
- From:
China Pharmacy
2015;26(36):5152-5154
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method to determinate the content of paeoniflorin in Bailing tablet,and to evaluate the uncertainty of results. METHODS:HPLC was performed on the column of Platisil C18 with mobile phase of water- acetonitrile(87:13,V/V) at a flow rate of 1 ml/min,the detection wavelength was 230 nm,the column temperature was 25 ℃ and the injection volume was 10 μl. Quantitative analysis was conducted for the processes and procedures and the uncertainty was evaluated. RESULTS:The linear range of paeoniflorin was 0.100 1-2.502 8 μg;RSDs of precision,stability and reproducibility tests were lower than 2.0%,recovery rate was 98.7%-100.9%(RSD=0.86%,n=6). Reference substance amount,elution produce and the accuracy of method had great effect on the uncertainty. CONCLUSIONS:The established method can be used for the content determination of paeoniflorin in Bailing tablet,and the uncertainty evaluation provides reliability for the method.
