Study on the Quality Standard of Liyan Mixture
10.6039/j.issn.1001-0408.2015.36.36
- VernacularTitle:利咽合剂的质量标准研究
- Author:
Xia JING
;
Jin XU
;
Fang SUN
;
Wen ZHAN
- Publication Type:Journal Article
- Keywords:
Liyan mixture;
Quality standard;
Scrophularia ningpoensis;
Terminalia chebula;
Glycyrrhiza uralensis;
TLC;
Chlorogenic acid;
HPLC
- From:
China Pharmacy
2015;26(36):5141-5143
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the quality standard of Liyan mixture. METHODS:TLC was conducted to identify the Scrophularia ningpoensis,Terminalia chebula and Glycyrrhiza uralensis;HPLC was conducted to determine the content of chloro-genic acid. The column was Agilent Eclipse Plus C18 with mobile phase of methanol-0.2% phosphoric acid (18:82,V/V) at flow rate of 1.0 ml/min,the detection wavelength was 327 nm,column temperature was 30 ℃ and the injection volume was 20 μl. RE-SULTS:TLC showed S. ningpoensis,T. chebula and G. uralensis had clear spots and good separation. There was no interference for negative control. The linear range of chlorogenic acid was 0.16-1.6 μg(r=0.999 9);RSDs of precision,stability and reproduc-ibility tests were lower than 1.0%,recovery was 96.9%-101.4%(RSD=1.17%,n=9). CONCLUSIONS:The method is simple and reproducible,and can be used for the quality control of Liyan mixture.
