Simultaneous Determination of Andrographlide and Dehydroandrographolide in Ganmaoqing Tablet by HPLC
10.6039/j.issn.1001-0408.2016.12.38
- VernacularTitle:HPLC法同时测定感冒清片中穿心莲内酯和脱水穿心莲内酯的含量
- Author:
Huiying XU
;
Baihong XU
;
Haoan CHEN
;
Yang LI
;
Danting SHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Ganmaoqing tablet;
Andrographlide;
Dehydroandrographolide
- From:
China Pharmacy
2016;27(12):1699-1701
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of andrographlide and dehydroandrographolide in Ganmaoqing tablet. METHODS:HPLC was performed on the column of Thermo BDS C18 with mobile phase of mathanol-water at a flow rate of 1.0 ml/min,detection wavelength was 225 nm for andrographlide and 254 nm for dehydroandrographolide,col-umn temperature was 30 ℃,and injection volume was 10 μl. RESULTS:The linear range was 2.25-45.00 μg/ml for andrographlide (r=0.9998) and 1.00-20.00 μg/ml for dehydroandrographolid(r=0.9990);RSDs of precision,stability and reproducibility tests were no more than 0.8%;average recoveries were 96.96%-99.80%(n=9) and 96.79%-100.65%(n=9). CONCLUSIONS:The method is simple,accurate and reliable,and can be used for the quality control of andrographlide and dehydroandrographolide in Ganmaoqing tablet.
