Simultaneous Determination of 4 Ginsenosides in Shengmai Powder by HPLC
10.6039/j.issn.1001-0408.2016.12.39
- VernacularTitle:HPLC法同时测定生脉散中4种人参皂苷类成分的含量
- Author:
Hua PING
- Publication Type:Journal Article
- Keywords:
HPLC;
Shengmai powder;
Ginsenoside Rb1;
Ginsenosides Rc;
Ginsenosides Rb2;
Ginsenosides Rd
- From:
China Pharmacy
2016;27(12):1702-1704
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination for ginsenoside Rb1,ginsenosides Rc,ginsen-osides Rb2 and ginsenosides Rd in Shengmai powder. METHODS:HPLC was performed on the column of Agilent ZORBAX Ex-tend-C18 with mobile phase of acetonitrile-0.1% formic acid(gradient elution) at a flow rate of 1.0 ml/min,wavelength was 203 nm,column temperature was 30 ℃ and the volume injection was 10 ml. RESULTS:The linear was 0.78-38.75 μg for ginsenoside Rb1(r=0.9996),0.51-25.50 μg for ginsenosides Rc(r=0.9996),0.43-21.50 μg for ginsenosides Rb2(r=0.9997)and 0.20-10.00 μg for ginsenosides Rd(r=0.9997);RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 96.6%-101.2%(RSD=1.6%,n=6),97.0%-102.1%(RSD=1.7%,n=6),99.1%-102.8%(RSD=1.3%,n=6)and 96.3%-101.1%(RSD=1.6%,n=6). CONCLUSIONS:The method is simple,rapid with good stability,reproducibility and high precision,and can be used for quality control of Shengmai powder.
