Simultaneous Determination of 3 Ingredients in Shilintong Tablet by HPLC
10.6039/j.issn.1001-0408.2016.12.42
- VernacularTitle:HPLC法同时测定石淋通片中3种成分的含量
- Author:
Hui LIU
;
Hua LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Shilintong tablet;
Vicexin 2;
Vicexin 1;
Schaftoside
- From:
China Pharmacy
2016;27(12):1710-1713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for simultaneous determination of vicexin 2,vicexin 1 and schaftoside in Shilin-tong tablet. METHODS:HPLC was performed on the column of Phenomenex C18 with mobile phase of methanol-0.1% phosphoric acid (gradient elution)at the flow rate of 0.8 ml/min,the detection wavelength was 334 nm,the column temperature was 25 ℃, and the injection volume was 10 μl. RESULTS:The linear range was 0.31-3.10 μg for vicexin 2 (r=0.9999),0.28-2.80 μg for vicexin 1(r=0.9999)and 0.39-3.90 μg for schaftoside(r=0.9999),respectively;RSDs of precision,stability and reproducibili-ty tests were lower than 3.0%;recoveries were 95.2%-100.4%(RSD=1.6%,n=9),96.3%-100.8%(RSD=1.4%,n=9) and 95.7%-100.9%(RSD=1.7%,n=9),respectively. CONCLUSIONS:The method is simple,sensitive ,accurate,reliable and repro-ducible,and can be used for the quality control of Shilintong tablet.
