Study on Improvement of Quality Standard of Shuangshen Capsule
10.6039/j.issn.1001-0408.2016.12.31
- VernacularTitle:双参胶囊的质量标准提高研究
- Author:
Xiaomin CUI
;
Fang LI
;
Fan LI
;
Weifeng WANG
- Publication Type:Journal Article
- Keywords:
Shuangshen capsule;
Salvianolic acid B;
TLC;
HPLC
- From:
China Pharmacy
2016;27(12):1677-1679
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the quality standard of Shuangshen capsule. METHODS:TLC was conducted for the qual-itative identification of Curcumnae radix and Rehmannia glutinosa in the preparation. HPLC was used for the content determination of salvianolic acid B in the preparation:the column was Diamonsil C18(2)with mobile phase of acetonitrile-0.1% H3PO4(22:78, V/V) at a flow rate of 0.5 ml/min,detection wavelength was 286 nm,column temperature was 30 ℃,and the injection volume was 2 μl. RESULTS:The TLC spots of C. radix and R. glutinosa were clear and well separated;the linear range of salvianolic ac-id B was 0.0792-0.792 μg(r=0.9999);RSDs of precision,stability and reproducibility tests were lower than 1%;recovery was 100.71%-101.82%(RSD=0.50%,n=6). CONCLUSIONS:The established standard can be used for the quality control of Shuang-shen capsule.
