Effects of Quercetin on Human Lung Cancer NCI-H1395 Cell Apoptosis
10.6039/j.issn.1001-0408.2015.34.11
- VernacularTitle:槲皮素对人肺腺癌NCI-H1395细胞凋亡的影响
- Author:
Lin LI
;
Dijin WANG
;
Ke WAN
;
Guohui XU
- Publication Type:Journal Article
- Keywords:
Quercetin;
Human lung cancer NCI-H1395 cell;
Cell apoptosis;
Caspase
- From:
China Pharmacy
2015;(34):4786-4788
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of quercetin on human lung cancer NCI-H1395 cell apoptosis. METHODS:CCK-8 was used to detect the effects of 0-200 μmol/L quercetin on human lung cancer NCI-H1395 cell proliferation after treated for 12,24 and 48 h. Hochest33258 staining and flow cytometry were used to detect the effects of 0,20,50,100 μmol/L quercetin on NCI-H1395 cell apoptosis after treated for 24 h. The effects of 100 μmol/L quercetin on NCI-H1395 cell apoptosis was investi-gated after treated with Caspase-8,Caspase-9,Caspase-3 inhibitor. RESULTS:Quercetin could inhibit NCI-H1395 cell prolifera-tion in dose and time-dependent manner. 20,50,100 μmol/L quercetin could induce the apoptosis of NCI-H1395 cell,and apoptot-ic rates were (18.6 ± 4.1)%,(39.1 ± 4.5)% and (58.2 ± 3.5)%. Caspase-8 and Caspase-3 activation inhibition could obviously weaken the inhibitory effects of quercetin on cell(P<0.05). CONCLUSIONS:Quercetin can inhibit NCI-H1395 cell proliferation and induce cell apoptosis,which is related to the external way of cell apoptosis through activating Caspase-8 and Caspase-3.
