Determination of Voriconazole in Human Plasma by HPLC
10.6039/j.issn.1001-0408.2015.32.13
- VernacularTitle:HPLC法测定人血浆中伏立康唑的浓度
- Author:
Youlin WANG
;
Lulu ZHOU
;
Lingfei YANG
- Publication Type:Journal Article
- Keywords:
Voriconazole;
HPLC;
Therapeutic drug monitoring
- From:
China Pharmacy
2015;(32):4501-4503
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a rapid,sensitive,accurate and stable method for the determination of voriconazole in human plasma in order to monitor clinical use of voriconazole. METHODS:HPLC-UV detection method was applied using carba-mazepine as internal standard. Plasma samples were treated with acetonitrile protein precipitation. The determination was performed on Phecda C18 column with 20 mmol/L monopotassium phosphate buffer solution (pH 6.0)-acetonitrile (50∶50,V/V) as mobile phase at flow rate of 1 ml/min. The column temperature was 40℃ and detection wavelength was 255 nm. The injection volume was 50 μl. RESULTS:The retention time of voriconazole and internal standard were 8.34 and 6.24 min. The linear range of voricon-azole in plasma were 0.10-20.00 μg/ml(r=0.999 5). The lowest limit of quantitation was 0.05 μg/ml. Intra-day and inter-day RSD were below 1.57% and 1.45%,respectively. The extraction recovery of low,medium and high concentrations were between 81.40%to 128.29%. CONCLUSIONS:The method is simple and accurate for therapeutic drug monitoring(TDM)of voriconazole.
